Projects of the College

DIPSAR strives to extend the horizons and explore the unimaginable potential of pharmaceutical sciences for the benefit of human race by giving prime importance to research. Students from M. Pharm. and Ph. D. are involved in various research projects

Current Ph.D. Research Projects

Synthesis of Some Heterocyclic Compounds as Potential Anticancer Agents
Chief Investigator: Prof. B. P. Srinivasan
Co Investigator: Prof. M.R. Yadav
Research Scholar: H. Yogish Kumar
Drug discovery is a complex and lengthy endeavor. Many strategies exist to acceleratetarget to clinical candidate selection, as well as to provide the highest quality candidate. Several lead finding strategies include the use of accumulated information for ligands of previously executed discovery programs, such as pharmacophore modeling, QSAR, 3D-QSAR. Protein kinases catalyze the phosphorylation of tyrosine and serine/threonine residues in various proteins involved in the regulation of all functions. Protein kinases can be broadly classified as receptor (e.g., EGFr, c-erbB2, PDGFR, and VEGFR2) or non-receptor (e.g.,c-src and b-raf) kinases. Inappropriate or uncontrolled activation of many of these kinases, by overexpression, constitutive activation, or mutation, has been shown to result in uncontrolled cell growth. However, despite the good hematological and cytogenetic responses obtained, primary refractory disease and secondary resistance still occur with drugs used in CML. Therefore, it is evident for need of improving efficacyof tyrosine kinase inhibitors. Keeping the above point in focus, we planned to synthesis various heterocyclic compounds for improved anticancer activity against cancer cell lines.

Investigation of New Pharmacological Intervention for Diabetic Cardiomyopathy inStreptozotocin induced NIDDM Rats

Chief Investigator: Prof. B. P. Srinivasan
Research Scholar: Raman Sharma
Diabetic cardiomyopathy refers to a disease process which affects the myocardium in diabetic patients causing a wide range of structural abnormalities eventually leading to LVH [left ventricular (LV) hypertrophy] and diastolic and systolic dysfunction or a combination of these. The concept of diabetic cardiomyopathy is based upon the idea that diabetes is the factor which leads to changes at the cellular level, leading to structural abnormalities as outlined above.. The pathogenesis of diabetic cardiomyopathy is a chronic and complex process that is attributed to abnormal cellular metabolism and defects in organelles such as myofibrils, mitochondria, sarcoplasmic reticulum, and sarcolemma. Abnormal calcium handling in the diabetic heart was found to be an early indicator of abnormal metabolism. This change then leads to improper cardiac contraction and relaxation processes—anearly diastolic and later systolic dysfunction— and eventually heart failure. Diabetic cardiomyopathy not only has been attributed to hyperglycemia, but also is related to hyperlipidemia and inflammation. Although these pathogenic factors cause diabetic cardiomyopathy probably via different mechanisms their major contribution to diabetic cardiomyopathy is oxidative stress. We hypothesize that plants having the anti diabetic and antioxidant potential can decrease the progression of diabetic cardiomyopathy when therapy is initiated in early stages of diabetes by strict blood glucose control in these animals. The current study is aimed to evaluate the role of plants having antioxidant and anti diabetic potential in diabetic cardiomyopathy.

To Study Protective Effects of Bio-flavanoids on Retinal Neurovascular Degeneration inDiabetic rats

Chief Investigator- Prof. B. P. Srinivasan
Co- investigator- Dr. Rohit Saxena (AIIMS)
Research Scholar- Binit Kumar
Diabetes Mellitus is potentially devastating disease with high morbidity and mortality. It is associated with number of complications that are principally vascular in nature. Diabetic microvascular pathology (i.e. Diabetic Retinopathy, DR) is a leading cause of blindness, end stage renal disease and variety of debilitating neuropathies. For the therapy of DR, currently available drugs are not effective in preventing DR but they can certainly effect the disease progression to some extent. For many decades there has not been any significant achievement in the medical management of diabetic retinopathy. Various intravitreous and systemic drugs has come up in the clinical phase like corticosteroids (Anecortave acetate, Dexamethasone, Fluocinoloneacetonide, Triamcinolone acetonide), anti-VEGF (Bevacizumab, Ranibizumab, Pegaptanib) and Protein Kinase C inhibitors (Epalrestat, Ranirestat) but have not turned out with a complete promise to treat the disorder. Therefore, there is continous search for safe and effective drugs for the management of DR. Plant derived drugs are expected to provide extra advantage of producing hypoglycemia in addition to inhibit all the proposed mechanism of DR etiology.

Identification, Isolation, characterization andbiological evaluation of some herbal drugs

Chief Investigator- Dr. Sharad Wakode
Research Scholar- Sakshi Bajaj
In the Western world, as the people are becoming aware of the potency and side effects of synthetic drugs, there is an increasing interest in the natural products, remedies with a basic approach towards the nature. All plants are well known drugs in the Indian system of medicines for their potential phytochemical and therapeutic values. Keeping in view the potential of these plants, an effort is made to standardize and evaluate these herbal drugs. Further, the drugs are selected on the basis of their proposed biological potencyand easy availability.

Protransfersome gel (PTG) for combination therapy: Formulation Optimization andPerformance Evaluation
Chief Investigator: Dr. Meenakshi K. Chauhan
Co-investigator: Dr. P. K. Sahoo
Research scholar: Ashwani Singh Rawat
Limited aqueous solubility of BCS Class II drugs leads to restricted bioavailability for these candidates. Transdermal drug delivery is an attractive and accessible route for systemic delivery of such active molecules, as it not only bypass the hepatic first pass effect but also provides controlled release. Other than these advantages it delivers the improved patient compliance and withdrawal of side effects. Many approaches have been developed to either destroy or fluidize the lipid bilayers, thereby,enhancing the penetration of drugs but vesicular systems have received widespread attention in the transdermal delivery field because of many intrinsic properties, like biodegradability, biocompatible in humans, amphiphilic nature, possibility to modulate drug bioavailability and ability to incorporate both hydrophilic and lipophilic drugs. Vesicular drug delivery system such as liquid crystalline pro-ultraflexible lipid vesicles “protransfersomes” that will be converted into the ultraflexible vesicles transfersomes in-situ by absorbing water from the skin. This system is more stable having higher entrapment efficiency, can be used as self-penetration enhancer, easy to scale up, better for dermal and transcutaneous delivery. This carrier comprises the advantage of unique elasto-mechanical characteristics of transfersomes that allow them to squeeze through the small pores across the skin, and hence affect into fast as well as enhanced drug permeation. Encouraged with the potential of the concept of protransfersome gel for the enhancement of transdermal drug permeation, it was envisaged to solve the problem of transdermal delivery of BCS Class II combination using this novel carrier system.

Management of Diabetes & its complications by Herbal Drugs in Streptozotocin induced rat model
Chief Investigator: Dr. Rajani Mathur
Co-investigator: Prof. S. K. Gupta
Research Scholar: Mr. Shirish S. Dongare
Diabetes mellitus (DM) represents a range of metabolic disorders characterized by hyperglycemia resulting from insulin deficiency or insulin resistance or both. Hyperglycemia, the primary clinical manifestation of diabetes, is strongly associated with development of the diabetic complications. Complications caused by hyperglycemia involve damage to the small vessels such as in neuropathy, nephropathy, and retinopathy, and large blood vessels as in cardiovascular diseases. Plants have always been an exemplary source of drugs and many of the currently available drugs have been derived directly or indirectly from them. A wide array of plant derived active principles representing numerous chemical compounds has demonstrated activity consistent with their possible use in the treatment of diabetes mellitus and its complications. The current study is aimed to find out therapeutic potential drugs for the prevention of Diabetes and its complications in experimental models and to study their effects are studied on several biochemical parameters like blood glucose, HbA1c, TNF-alpha,
VEGF, Aldose reductase, total antioxidant etc. and histopathological studies will be carried out.

Pharmacological screening of some Indian medicinal plants for anticancer and
antiangiogenic activities

Chief Investigator: Dr. Rajani Mathur
Research Scholar: Mrs. S. Latha
Cancer is the uncontrolled growth and spread of cells. It can affect any part of the body. Cancer is a leading cause of death worldwide and accounted for 7.6 million deaths in 2008 alone (around 13%of all deaths).Angiogenesis is the formation of new capillaries from pre-existing vascular network and plays an important role in tumor growth and metastasis. New blood vessels are considered to be essential for the delivery of nutrients and oxygen to the tumor microenvironment, and provides route for metastasis, leading to high mortality, poor prognosis and drug resistance.The antiangiogenic strategy aims to develop compounds that can control tumor growth by blocking its ability to develop a blood supply. In the present study, an attempt will be made to screen some Indian medicinal plants for their anticancer and antiangiogenic activities.

Current M. Pharm. ResearchProjects

Development and Validation of RP-UFLC method for Simultaneous Quantitation of Mometasone furoate and Formoterol fumarate inPharmaceutical dosage forms.
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Anoosha
The present study deals with the development and validation of RP-UFLC method for simultaneous analysis of two poorly watersoluble drugs viz. Mometasone furoate and Formoterol fumarate dihydrate in pharmaceutical dosage forms. The combination of Formoterol fumarate and Mometasone furoate has been approved by the USFDA for treatment of asthma. While Mometasone furoate is a corticosteroid demonstrating potent anti-inflammatory activity, Formoterol fumarate dihydrate is a long-acting selective beta2-adrenergic receptor agonist that acts locally in the lungs as a bronchodilator. Literature survey reveals that currently there is no RPUFLC method for simultaneous quantitation of Formoterol fumarate and Mometasone furoate in pharmaceutical dosage forms although these drugs have been estimated alone in formulations using HPLC technique. Thus, the aim of present research work is to develop a sensitive as well as quantitative UFLC method for simultaneous quantitation of two anti-asthmatic drugs such that both the drugs elute as sharp characteristic peaks in UFLC chromatogram with sufficient resolution and separation.

To study the effects of Fucosterol in a rat model of experimental streptozotocin induced diabeticretinopathy.
Chief Investigator: Dr. B.P. Srinivasan
Research Scholar: Mohd Tariq Roomi
Diabetes is a metabolic disorder of multiple aetiology characterized by chronic hyperglycaemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action, or both. Diabetic patient is susceptible to a wide variety of chronic complications including diabetic retinopathy which is an ocular manifestation of diabetes. It can be defined as progressive dysfunction of the retinal blood vessels caused by chronic hyperglycemia. It is a leading cause of blindness in developed countries and affects up to 80 percent of all patients who have had diabetes for 10 years or more. After 20 years of diabetes, nearly 99% of patients with type 1 diabetes and 60% with type 2 have some degree on diabetic retinopathy. It is asymptomatic in early stages of the disease, if not treated though it can cause low vision and blindness. Fucosterol is a crystalline solid, commonly present in plants belonging to family Fucaceae. It has beneficial effects, including antioxidant, antidiabetic, anti inflammatory and ACE inhibitory activities. In the present study, its effects on various biochemical parameters like blood glucose, insulin, TNF-α, VEGF will be measured and its activity against Diabetic Retinopathy will be evaluated.

Standardization and effect of kigelia pinnata( leaves)extract in Streptozotocin induced ratmodel for diabetic nephropathy.
Chief Investigator: Prof. B.P.Srinivasan
Research scholar: Ritika Sharma
Diabetic nephropathy (DN) is a major microvascular complication of diabetes, representing the leading cause of ESRD. Clinical hallmarks of diabetic nephropathy include a progressive increase in urinary albumin excretion and a decline in the glomerular filtration rate. Leaves of kigelia pinnata commonly known as sausage tree was extracted using ethanol as a solvent and the whole extract was administered at a dose of 200 and 400mg/kg to STZ induced diabetic two day old neonatal wistar albino rats. Biochemical parameters such as albumin, creatinine, urea, TGF β, total protein, TNFα and oxidative stress parameters like lipid peroxidation(MDA), Catalase were measured.

Antibiotic Eluting Scaffolds for Wound Healing and Related Tissue Engineering Application
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Jatin Gupta
Wound healing process is greatly influenced by the microbial load at the wound site. High microbial load mostly lead to inflammation and abscess formation. Microbial load at the wound sites has to be reduced to fasten the healing process. Antibiotics have been the most suitable choice for curtailing bacterial growth. However, most of the antibiotics that are taken orally lead to numerous systemic side effects.One of the solutions to this condition is localized delivery of antibiotics which can be achieved by designing drug eluting implants that will help to decrease in-situ bacterial load. Localized and site specific delivery will help to maintain suitable concentrations of the drug as well as prevent any systemic toxicity. Long term localized delivery of antibiotics can be achieved by development of drug eluting implants like scaffolds or hydrogels. This study aims at optimization of encapsulation and release of model antibiotics like gentamycin and neomycin into Polylactic acid microparticles formulated by double emulsion solvent evaporation method. These microparticles have a property of fusion on treatment with methanol to form an interconnected porous scaffold. The resultant scaffold aids as a drug eluting implant and can deliver drug up to 30 days. Moreover growth factors helping in wound healing can also be encapsulated in these scaffolds. Application of such scaffold would augment the wound healing process by providing a adhering substrate for proliferating host cells as well as reducing the bacterial load. Polylactic acid being biocompatible and bioresorbabale, this scaffold would prove a promising device to addresstherapeutic wound healing.

To study the effect of Curculigo orchioides root extract in an experimental rat model ofstreptozotocin induced diabetic nephropathy
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Vasudha
Diabetes is a metabolic disorder characterized by hyperglycemia and leads to various microvascular complications like nephropathy. Diabetic nephropathy is the most common cause of end-stage renal disease. The clinical evidences are microalbuminuria followed by continuous increase in urinary protein excretion and declining glomerular filtration rate. The imbalances in metabolic factors (advanced glycation end products formation, increased protein kinase c activity) and haemodynamic factors (activation of renin-angiotensin-aldosterone system) are responsible for development of diabetic nephropathy. Curculigo orchioides is used traditionally as a diuretic, tonic, anti-infective and healing agent as well as in the treatment of jaundice, diarrhea, piles etc. The aim of the present study is to see the effect of the drug extract in a neonatal rat model of streptozotocin-induced diabetic nephropathy. Various biochemical parameters like blood glucose, insulin, TNF-α, TGF-β will be measured along with albumin, creatinine in diabetic control as well as treated rats.

To study the effect of Benincasa hispida(seed) extract in streptozotocin induced rat model fordiabetic cardiomyopathy.
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Shweta Sharma
Diabetic cardiomyopathy, a prominent cardiovascular complication has been recognized as a microvascular disease that may lead to heart failure. This disorder is a complex diabetes-associated process characterized by significant changes in the physiology, structure and mechanical function of heart. Pathogenesis of diabetic cardiomyopathy involves vascular endothelial cell dysfunction, as well as myocyte necrosis. Clinical trials have identified hyperglycemia as the key determinant in the development of chronic diabetic complications. Sustained hyperglycemia induces several biochemical changes including increased nonenzymatic glycation, sorbitol-myoinositolmediated changes, redox potential alterations and protein kinase c (PKC) activation, all of which have been implicated in diabetic cardiomyopathy, other contributing metabolic abnormalities may include defective glucose transport, increased myocyte fatty acid uptake and dysmetabolism. The current study is aimed to evaluate the effect of Benincasa hispida (seed) extract in diabeticcardiomyopathy.

Preparation and evaluation of solid dispersion ofcarvedilol
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Sidh Raj
Solid dispersions in water-soluble carriers are means of improving the dissolution rate, and hence possibly bioavailability, of a range of hydrophobic drugs. The poor solubility of carvedillol leads to poor dissolution and hence variation in bioavailability. The purpose of the present work is to increase the solubility and dissolution rate of carvedilol for enhancement of oral bioavailability. In the present work solid dispersions with PVP K30 and different grades of PEG are to be prepared by solvent evaporation method. The physical mixture and solid dispersion(s)are characterized for drugcarrier interaction, drug content, solubility and dissolution rate.

Starting with exports in pharmaceutical industry
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Geet
Objective:To understand perceptions of patients regarding Hormonal treatment options in prostate cancer patients. The objective is to understand the real world emotions of patients and identify unmet needs. Methodology: Research to be conducted through publicly available patient forums, where prostate cancer patients are networking with each other and discussing about their journey through the disease. Data to be mined through publicly available forums. No effort to be made to identify the patients or access any private discussions. The final report won’t have any mentions about the patients name, id and contact details and will completely adhere to privacy protection laws thatare applicable for any market research project.

Development and Validation Of Stability Indicating RP- HPLC, HPTLC and spectrophotometric method for the simultaneous estimation of CINNARIZINE
and DIMENHYDRINATE in tablet dosage forms.
Chief Investigator- Prof. B.P. Srinivasan
Research Scholar- Ms. Aastha Ahuja
The combination – Cinnarizine and Dimenhydrinate was approved for marketing in India in October,2010. Both the drugs belong to the class of anti – histaminics and the combination is prescribed as an anti-emetic for the treatment of severe vertigo. Cinnarizine is official in BP and EP , both of which include potentiometric titration for its estimation. Dimenhydrinate is official in BP. USP, EP and JP 15 , which include potentiometric and argentometric titrations and HPLC method for its estimation. The combination of these two drugs is not official in any pharmacopoeia. Literature review shows that numbers of analytical methods are available for estimation of both the drugs either alone or in with other drugs. Till date, there is no official analytical method for simultaneous estimation of both the drugs in their combined dosage form. Therefore, the objective is to develop a UV, HPTLC, RP-HPLC method for simultaneous estimation of Cinnarizine and Dimenhydrinate in their formulation and to validate the developed method according to ICH guidelines.

Development and validation of UV Method, Stability indicating RP-HPLC Method and HPTLC Method for the simultaneous estimation of Eperisone HCl and Diclofenac sodium in bulk drugs and combined capsule dosage form.
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Archana
This combination of Eperisone HCl and Diclofenac sodium prescribed for the treatment of patients with acute musculoskeletal spasm associated with low back pain .Available formulation is EPRY D SR capsules (EISAI). Eperisone HCl, 4-ethyl-2-methyl-3-piperidinopropiopenone, a well known antispasmodic drug official in Japanese Pharmacopoeia. JP describes potentiometric method for its estimation. Literature survey reveals that Electronic Spray Ionization MS for determination of Eperisone in human plasma. The use of HPLC/MS, GC/ MS, NMR, UV & IR to identify degradation product of Eperisone HCl in tablets. Diclofenac sodium, 2-(2,6-dichlorophenylamine)benzene acetic acid sodium official in IP & BP which describes liquid chromatography method for its estimation. Literature survey reveals HPLC, UV & HPTLC for determination of Diclofenac in single dosage form and in combination with other drugs. This combination is not official in any Pharmacopoeia, hence no official method is available for simultaneous estimation of Eperisone HCl and Diclofenac sodium in dosage forms. Considering this, the present study aims to develop validated methods for their simultaneous estimation in pharmaceutical formulations.

Solubility Enhancement of Felodipine by Solid Dispersion technique, its Characterization and invitro evaluation
Chief Investigator: Prof. B.P. Srinivasan
Research Scholar: Shubhika Kwatra
In biological systems, drug dissolution in an aqueous medium is an important prior consideration for systemic absorption. Increasing the solubility and dissolution rate of drugs is a significant challenge to pharmaceutical scientists as bioavailability depends largely on these factors. Solid dispersion technique is one of the effective and accepted techniques for enhancing drug solubility. Felodipine, a Calcium Channel blocking agent, is classified under BCS Class- I. It is practically insoluble in water and hence,there is a need to enhance the aqueous solubility of Felodipine. In the present study, solid dispersions of Felodipine are being prepared by melt method and solvent evaporation method using different hydrophilic carriers in various drug-carrier ratios. The prepared dispersions will be evaluated for drug content, solubility and in vitro release. The physical state and drug: polymer interactions of solid dispersions and physical mixtures will be characterized by Scanning Electron Microscopy, X-ray diffraction, Differential Scanning Calorimetry and Fourier Transform Infrared Spectroscopy.

 

Development and Validation Of Stability Indicating RP- HPLC, HPTLC and spectrophotometric methodfor the estimation of ILAPRAZOLE in tablet dosage forms.
Chief Investigator Prof. B.P. Srinivasan
Research Scholar: Ms. Surbhi Rohatgi
Ilaprazole,a proton pump inhibitor was approved for marketing in India in April,2011. It is indicated for treatment of dyspepsia, peptic ulcer disease (PUD) and gastro-esophageal reflux disease (GERD). Literature survey reveals HPLC method for determination of Ilaprazole and its metabolites in human plasma. No method is reported for quantification in pharmaceutical dosage form. The study aims at developing a sensitive, accurate, precise and robust UV, HPLC and HPTLC method for the determination of Ilaprazole in marketed tablets. The validation is performed as per ICH guidelines.

Understanding and reducing the medicationerrors in tertiary hospital in India
Chief Investigator Prof. B.P. Srinivasan
Research Scholar: Vaibhav Gupta
Background The primary goal of reducing medication errors should be to eliminate those that reach the patient. Objective To study the pattern of error interceptions in the medication use process. Setting Tertiary care hospital in India. Method The ‘Swiss Cheese Model’ was used to explain the interceptions targeting medication error reporting over 5 months (February–July). Main outcome measures The prescribers in the prescribing stage, pharmacy staff members at the drug dispensing stage and nurses at the drug administration stage were considered as defensive layers in the medication use process. Hence, the proportions of prescribing, dispensing and drug administration errors that reached the patient was used a measure. Conclusion Although prescribing errors are still the most frequent error type, most are detected, whilst most drug administration errors still reach the patient. As drug administration errors affect patients directly, introducing defensive layers such as clinical pharmacy services or technological interventions between the drug administration process and the patient may greatly help to prevent drug administration errors from reaching the patient.

Solid phase synthesis of disulphide loop peptide using fmoc chemistry and its purification by rp-hplc
Chief Investigator: Dr. D.P. Pathak
Research Scholar: Supriya Nagori
Synthetic peptides are valuable tools in analysis of naturally occurring peptides or proteins. Solid-support synthesis is now the most widely used method to prepare synthetic peptides. The advantages of solid-support synthesis are its speed, versatility, ease of automation and low costs. RP-HPLC is used to purify the peptide sample on the basis of their polarity. The present study was aimed to optimize the loading of amino acid on resin, effect of various nucleophilic reagents during cleavage of peptide from resin(TFA :TIS : water), effect of oxidation on thiol group containing amino acids, effect of combined strategy of Boc & Fmoc synthesis and optimize the purification of hydrophobic peptide by RPHPLC.

Phytochemical and pharmacognostic evaluation of Bauhinia variegata linn var. Candida.
Chief Investigator:- Mrs. Manju Vyas Singh
Research Scholar:- Akhilesh Kr. Gond
Herbs and Shrubs have been used from time immemorable for their medicinal properties. Diseases had haunted even our ancestors and through trial and error method they used things available in the nature for curing them. In the process herbs and shrubs were found to have medicinal properties. In a need to prove the traditional uses of medicinal plants scientific studies is required. There is always a scope to find out better drugs with higher therapeutic utility and least side effects, even though almost all diseases are curable with existing drugs. Bauhinia variegate linn var. candida . is widely used in folklore medicine. It’s bark, root, leaves, seeds and flowers are used for their medicinal properties. It has been used in dyspepsia, to prevent obesity, as an astringent, tonic, etc. It is found to contain tannins, sterols and flavanoids. In the present study we aim to standardize the plant, do the phytochemical investigation, then isolate active constituents from the bark of the plant and to characterize them using techniques like NMR, Mass etc.

Phytochemical and pharmacognostic evaluation of jacaranda species.
Chief Investigator:- Mrs. Manju Vyas Singh.
Research Scholar:- Meenakshi.
Plants have formed the basis for traditional medicinal systems for thousands of years , with the first records dating from about 2600 BC in Mesopotamia, Ancient Egyptian , Chinese and Indian documents show that medicines in these societies included numerous plant based remedies. The single molecule based drugs used today can have serious side effects. Hence there is a steady emergence of preference for herbal preparations, particularly if they are effective as single molecule. In the present study we aim to standardize JACARANDA SPECIES plant, isolate active constituents and characterize them using spectroscopic techniques.

Bioavailability enhancement of a glycopeptides drug by provesicular approach
Chief Investigator: Dr. Meenakshi K. Chauhan
Research scholar: Anubha Mahajan
Vancomycin (VCM) is a glycopeptide antibiotic which is used for the treatment of serious life-threatening infections by Gram-positive bacteria that are unresponsive to other less toxic antibiotics. It is used for the treatment of MRSA, MRSE, prophylaxis of endocarditis. It is a BCS class III drug with high solubility and low permeability. Since it has poor oral bioavailability, it has been given intravenously for most infections and is never been taken as a first line treatment of Staphyloccus aureus. So, this project aims at enhancing the oral bioavailability of Vancomycin. In this, lipophilic complex of the drug is formed and complex is incorporated into the lipid layer by film hydration method. In order to increase the gastrointestinal (GI) absorption of VCM, we have focused on a novel carrier system.

Oral bioavailabiltiy enhacement by novel provesicular approach
Chief Investigator: Dr. Meenakshi K. Chauhan
Research Scholar: Aruna Singh
The aminoglycoside antibiotic, gentamicin (GM), is an important antibacterial agent used for the treatment of a wide variety of gram-negative bacilli and gram-positive cocci infections. Because GM is a polarized watersoluble compound, it is commonly recognized that intestinal membrane permeability is poor resulting in low bioavailability (BA). Since GM is not adequately absorbed after oral administration, the clinical use of GM is limited to injection or topical dosage forms. However, a number of problems are associated with the use of GM in injection form, including the inconvenience of injections for long-term administration. Parenteral administration of GM has been associated with side effects that include mainly nephrotoxicity and ototoxicity. Therefore, attempts have been made to develop an alternative route of administration, such as through the nasal mucosa for systemic therapy, which would improve QOL in patients. However, it was also observed that aminoglycoside antibiotics such as GM have poor transmucosal BA. In order to increase the gastrointestinal (GI) absorption of GM, we have focused on a novel carrier system.

To record the effect of aqueous leaf extract of Psidium guajava on isolated tissue preparations and elucidate its interaction with Cholinergic, Serotonergic and Histaminergic Systems.
Chief Investigator: Dr. Rajani Mathur
Research Scholar: Rakesh Kumar Mahaseth
Psidium guajava is an important food crop and medicinal plant available in tropical and subtropical countries, widely used in food and folk medicines around the world. Psidium guajava mainly known for its antispasmodic and antimicrobial properties is used in the treatment of diarrhea and dysentery. It has also been used extensively as hypoglycemic agent. Many pharmacological studies have demonstrated its ability for anti-cough, antidiabetic, anti-inflammatory and anti-nociceptive activities. However, the interaction of aqueous leaf extract of Psidium guajava with Cholinergic, Serotonergic and Histaminergic Systems has not been studied till now. The present study aims to determine the affinity of aqueous leaf extract of Psidium guajava for Cholinergic, Serotonergic  and Histaminergic receptor systems using various rat tissue preparations and comparing them with standard receptor agonists and antagonists.

To record the effect of aqueous leaf extract of Aegle marmelos on isolated tissue preparations and elucidate its interaction with Cholinergic,Serotonergic and Histaminergic systems.
Chief Investigator: Dr. Rajani Mathur
Research Scholar: Sanjeev Kumar
Aegle marmelos is recognized in traditional medicine for the treatment of various diseases such as dysentery, fever, diabetes, asthma, heart problems, ophthalmic, hemorrhoids and urinary problems in humans. The leaf part of the plant has been claimed to be used for the treatment of irritable bowel syndrome, inflammation, asthma, fever, hepatitis and as an analgesic. The aqueous extract of leaves is beneficial in the treatment of leucorrhoea, conjunctivitis and deafness. However, the interaction of aqueous leaf extract of Aegle marmelos with Cholinergic, Serotonergic and Histaminergic Systems has not been studied till now. The present study aims to determine the affinity of aqueous leaf extract of Aegle marmelos for Cholinergic, Serotonergic and Histaminergic receptor systems using various rat tissue preparations and comparing them with standard receptor agonists and antagonists.

Standardization of achyranthes aspera and nephroprotective activity of oleanolic acid formulation using albino wistar rat .
Chief Investigator: Mr. P.N. Raju
Research Scholar: Bharat Bhati
Standardization of Achyranthes Aspera will be done according to WHO guidelines using oleanolic acid as marker compound. Isolated oleanolic acid will be evaluated for nephroprotective activity using cisplatin induced nephrotoxicity in albino wistar rats using biochemical and histopathological parameters.